Localization, Accumulation, and Antifungal Activity of Chitinases in Rye (Secale cereale) Seed
نویسندگان
چکیده
منابع مشابه
Localization , Accumulation , and in Rye ( Secale cereale ) Seed AntifungalActivity of Chitinases
In order to understand a physiologi¢ al role of chitinases in rye, the localization and accumulatien of rye seed chitinase-a and -c (RSC-a and -c) in the seeds were studied by imm"nochemical methods. An antiserum specific to the chitin-binding domain (CB-domain), whicb is an N-terminal part of RSC-a, and an antiserum specific to the catalytic region of RSC-a and RSC-c were used. An immunoblot a...
متن کاملAntifungal Activity of Rye Binding Manner of Class I JSBA ( Secale cereale ) Seed Chitinases : the Different and Class II Chitinases to the Fungal Cell VValls
cell walls prepured from its mycelia. RSC-a inhibited . more strong}y the re-extension of the hyphae, containing mainly mature cells, than RSC-c did. Upon incubation of the fungus vvith fluorescent chitinases, FITC}abeled RSC-a was found to be located in the hyphal tips, lateral walls, and septa, whi]e FITC-labeled RSC-c was only in the hyphal tip. RSC-a had a greater aMnity for the cell walls...
متن کاملLocalization , Accumulation , and in Rye ( Secale
In order to understand a physiologi¢ al role of chitinases in rye, the localization and accumulatien of rye seed chitinase-a and -c (RSC-a and -c) in the seeds were studied by imm"nochemical methods. An antiserum specific to the chitin-binding domain (CB-domain), whicb is an N-terminal part of RSC-a, and an antiserum specific to the catalytic region of RSC-a and RSC-c were used. An immunoblot a...
متن کاملMolecular cloning, functional expression, and mutagenesis of cDNA encoding rye (Secale cereale) seed chitinase-c.
We cloned a complete cDNA encoding rye seed chitinase-c, designated RSC-c, by rapid amplification of cDNA end and PCR procedures. The cDNA of RSC-c consists of 1,018 nucleotides and includes an open reading frame encoding a polypeptide of 266 amino acid residues. A recombinant RSC-c was produced by expression in Escherichia coli Origami(DE3) and purified. rRSC-c had almost the same chitinase ac...
متن کاملTowards a whole-genome sequence for rye (Secale cereale L.).
We report on a whole-genome draft sequence of rye (Secale cereale L.). Rye is a diploid Triticeae species closely related to wheat and barley, and an important crop for food and feed in Central and Eastern Europe. Through whole-genome shotgun sequencing of the 7.9-Gbp genome of the winter rye inbred line Lo7 we obtained a de novo assembly represented by 1.29 million scaffolds covering a total l...
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ژورنال
عنوان ژورنال: Bioscience, Biotechnology, and Biochemistry
سال: 2001
ISSN: 0916-8451,1347-6947
DOI: 10.1271/bbb.65.2710